Learning path · Beginner
HPLC from zero
From what an HPLC is to running, calculating, calibrating and troubleshooting a real assay.
- 11 steps
- For: Students and new QC analysts
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- ReadWhat HPLC is and what it is used forThe big picture before the details.
- ReadWorking principle and main partsHow separation actually happens, part by part.
- ReadKey terms: retention time, resolution, tailingThe words every chromatogram is described with.
- ReadOperating the instrumentStart-up, sequence and shutdown in the right order.
- ReadA real method: Paracetamol tablets assaySample preparation, settings and system suitability in practice.
- CalculateWork out the assay yourselfTurn peak areas into % of label claim.
- CalculateCheck system suitability precisionThe %RSD every run must pass.
- ReadHow the HPLC is calibratedWhy your results can be trusted.
- ReadPump flow rate calibration in detailA complete calibration procedure, step by step.
- ReadTroubleshooting common problemsPressure, drift, tailing and ghost peaks.
- PractiseTest yourselfMCQs and viva questions with explanations.