Ultra-High Performance Liquid Chromatography (UHPLC)
A high-pressure form of HPLC that uses columns packed with very small particles to give faster runs, sharper peaks and less solvent use. Used in pharma QC for fast assays, impurity profiling and high-throughput testing.
- Pharmaceutical
- Biotech
- Contract research (CRO / CDMO)
- Food & beverage
- Environmental
- Clinical research
UHPLC calibration procedures
Pump flow rate accuracy
Checks that the UHPLC pump delivers the set flow by weighing the water collected over a timed interval.
Open procedureInjector precision
Checks that the UHPLC autosampler injects the same small volume every time, using replicate injections of a caffeine solution.
Open procedureDetector wavelength accuracy
Confirms the UHPLC UV / PDA detector reads the correct wavelength, using the built-in holmium oxide filter or a caffeine solution.
Open procedureInstrument qualification (DQ · IQ · OQ · PQ)
- 01
DQ — Design qualification
States the user requirements: pressure rating, dwell volume, flow range, injection range, detector type and cell volume, data rate and a compliant CDS.
- 02
IQ — Installation qualification
Confirms delivery, installation, utilities, firmware and software versions, and that the system is connected to the CDS correctly.
- 03
OQ — Operational qualification
Tests flow accuracy, gradient accuracy, injector precision, carry-over, detector wavelength accuracy, noise and linearity, and column temperature against specifications.
- 04
PQ — Performance qualification
Shows the system performs consistently for its intended methods, for example with routine system suitability and a periodic holistic test mixture.
Keep every UHPLC in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.