Chromatography
Ultra-High Performance Liquid Chromatography (UHPLC)
A high-pressure form of HPLC that uses columns packed with very small particles to give faster runs, sharper peaks and less solvent use. Used in pharma QC for fast assays, impurity profiling and high-throughput testing.
- Pharmaceutical
- Biotech
- Contract research (CRO / CDMO)
- Food & beverage
- Environmental
- Clinical research
PREVENTIVE MAINTENANCE
Maintenance checklist
Daily / each use
- Record system pressure at the start of the run
- Check needle-wash and seal-wash levels
- Check for leaks at the column fittings
- Replace mobile phases rather than topping up
Weekly
- Replace in-line filter elements if pressure has risen
- Clean solvent bottles and inlet filters
- Check the autosampler needle and seal for deposits
Monthly / quarterly
- Check lamp energy and hours
- Review pressure ripple and replace check valves or seals if it is rising
- Clean the flow cell if baseline noise increases
Six-monthly / annual
- Preventive maintenance by a trained engineer
- Calibration of flow, injector precision and detector wavelength
SAFETY
Safety precautions
Before you work on the UHPLC
- Solvent jets at UHPLC pressure can penetrate skin: never loosen fittings or touch leaks while the system is pressurised.
- Organic solvents are flammable and toxic; prepare mobile phases in a fume hood.
- Do not look directly at the UV lamp.
- Collect waste in closed, labelled containers and dispose of it as per site procedure.
- Wear safety glasses, gloves and a lab coat.
In LimsPulse · Calibration Management
Keep every UHPLC in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.