LimsPulse
Bioanalytical

Real-time PCR (qPCR) system

A thermal cycler with a built-in fluorescence reader. It copies a chosen DNA sequence cycle by cycle and measures the fluorescence in every well after each cycle, so the starting amount of DNA can be calculated from a standard curve.

  • Biotechnology
  • Biopharmaceutical
  • Cell and gene therapy
  • Academic research
  • Agricultural biotech
  • Food authenticity
Photo of a Real-time PCR (qPCR) system
Photo: İnformatic, CC BY-SA 3.0 (cropped), via Wikimedia Commons
CALIBRATION

qPCR calibration procedures

Block temperature uniformity verification

Checks that every well position of the qPCR block reaches the set temperature and that the block is even from well to well.

Open procedure

Optical (dye) calibration

Updates the optical calibration files (background, well uniformity and pure-dye spectra) and confirms the system then quantifies a reference DNA series correctly.

Open procedure
QUALIFICATION

Instrument qualification (DQ · IQ · OQ · PQ)

  1. 01

    DQ

    Define needs: plate format, number of dye channels, thermal ramp rate, uniformity specification, sensitivity, 21 CFR Part 11-capable software.

  2. 02

    IQ

    Check delivery, installation site (bench, ventilation, power), software installation, user accounts and documentation.

  3. 03

    OQ

    Verify block temperature accuracy and uniformity, heated lid temperature, optical calibrations (background, uniformity, dyes) and software functions including audit trail.

  4. 04

    PQ

    Run a reference standard dilution series with NTCs; confirm efficiency, linearity, Cq precision and absence of contamination meet set criteria.

In LimsPulse · Calibration Management

Keep every qPCR in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how