LimsPulse
Spectroscopy

Fluorescence Spectrophotometer (Spectrofluorometer)

Shines light of one wavelength on a sample and measures the longer-wavelength light the molecules give back (fluorescence). Very sensitive and selective for fluorescent compounds.

  • Pharmaceutical
  • Biotech
  • Clinical
  • Environmental
  • Food & beverage
  • Research
Photo of a Fluorescence Spectrophotometer (Spectrofluorometer)
Photo: VGV27, CC BY-SA 4.0 (cropped), via Wikimedia Commons
PREVENTIVE MAINTENANCE

Maintenance checklist

Daily / each use

  • Clean cuvettes
  • Check sample compartment for spills
  • Run a quick water Raman or reference check if required by SOP

Weekly

  • Wipe the sample compartment
  • Check the cuvette holder alignment

Monthly / quarterly

  • Check lamp hours and intensity
  • Inspect cuvettes for scratches

Six-monthly / annual

  • Wavelength accuracy and sensitivity calibration
  • Preventive maintenance by service engineer, including lamp replacement when needed
SAFETY

Safety precautions

Before you work on the Fluorimeter
  • Xenon lamps emit intense UV light and are under high pressure; replace them only when cold, wearing face protection.
  • Never look into the beam or sample compartment with the lamp on.
  • Handle organic solvents and fluorescent dyes with gloves; some dyes are toxic or mutagenic.
  • Dispose of quartz cuvettes and solvent waste as per site procedure.
In LimsPulse · Calibration Management

Keep every Fluorimeter in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how