Fluorescence Spectrophotometer (Spectrofluorometer)
Shines light of one wavelength on a sample and measures the longer-wavelength light the molecules give back (fluorescence). Very sensitive and selective for fluorescent compounds.
- Pharmaceutical
- Biotech
- Clinical
- Environmental
- Food & beverage
- Research
Fluorimeter calibration procedures
Wavelength accuracy
Checks that the excitation and emission monochromators read the correct wavelength, using sharp lines of known wavelength such as mercury emission lines.
Open procedureSensitivity (water Raman signal-to-noise)
Measures the Raman scatter band of pure water and compares it with the background noise. The ratio is a standard way to compare fluorimeter sensitivity.
Open procedureInstrument qualification (DQ · IQ · OQ · PQ)
- 01
DQ — Design qualification
Defines wavelength range, sensitivity, scan speed, temperature control and software compliance needs.
- 02
IQ — Installation qualification
Confirms correct installation, components, lamp, software version and documentation.
- 03
OQ — Operational qualification
Tests excitation and emission wavelength accuracy, sensitivity (water Raman signal-to-noise), repeatability and linearity.
- 04
PQ — Performance qualification
Shows routine fitness, e.g. a check standard or water Raman intensity tracked over time.
Keep every Fluorimeter in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.