LimsPulse
Microbiology & sterility

Endotoxin (LAL) reader

An incubating photometer that holds LAL reactions at 37 °C and measures the change in absorbance or turbidity over time. Software turns the reaction onset time into an endotoxin concentration in EU/mL using a standard curve.

  • Pharmaceutical
  • Biotech
  • Medical devices
  • Dialysis and hospital pharmacy
  • Research laboratories
CALIBRATION

Endotoxin reader calibration procedures

Temperature and photometric checks

Verifies that the reader holds all wells or tubes at 37 ± 1 °C and that its absorbance readings at 405 nm (or turbidity readings) agree with certified reference filters or plates.

Open procedure
QUALIFICATION

Instrument qualification (DQ · IQ · OQ · PQ)

  1. 01

    DQ — Design qualification

    Defines the reader type (plate or tube), methods (chromogenic, turbidimetric), wavelength, capacity, software and data integrity needs.

  2. 02

    IQ — Installation qualification

    Records the reader, computer, software version, user roles and calibration certificate.

  3. 03

    OQ — Operational qualification

    Verifies temperature accuracy and uniformity, photometric accuracy and linearity, read timing and software calculations.

  4. 04

    PQ — Performance qualification

    Runs standard curves and spiked samples to show the system meets USP <85> criteria, and qualifies each analyst with the method.

In LimsPulse · Calibration Management

Keep every Endotoxin reader in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how