LimsPulse
Microbiology & sterility

Endotoxin (LAL) reader

An incubating photometer that holds LAL reactions at 37 °C and measures the change in absorbance or turbidity over time. Software turns the reaction onset time into an endotoxin concentration in EU/mL using a standard curve.

  • Pharmaceutical
  • Biotech
  • Medical devices
  • Dialysis and hospital pharmacy
  • Research laboratories
Follow your site SOP
This is a typical operating sequence for learning. Your instrument model and approved SOP take precedence.
STAGE 1

Before the run

  1. 1Switch on the reader to warm up and reach 37 °C; check the temperature display.
  2. 2Confirm calibration status and that the software user has the right access.
  3. 3Bring LAL reagent, CSE and LAL reagent water (LRW) to room temperature; use depyrogenated glass and certified endotoxin-free tips and plates.
  4. 4Prepare the plate map: standards, negative controls, samples and PPCs, all in duplicate or as per SOP.
  • Endotoxin sticks to some plastics and is everywhere in the environment. Use only certified pyrogen-free consumables.
STAGE 2

Running the test

  1. 1Vortex the CSE as per the certificate and make the standard dilutions, vortexing each step.
  2. 2Pipette standards, samples and spikes into the plate and pre-incubate in the reader.
  3. 3Reconstitute the LAL reagent gently (do not vortex unless the supplier says so) and add it to all wells with a multichannel pipette quickly.
  4. 4Start the kinetic read immediately.
  • Bubbles in the wells disturb readings. Pipette carefully.
STAGE 3

Review

  1. 1Check that the standard curve meets the correlation criterion (|r| ≥ 0.980) and that the negative controls are below the lowest standard.
  2. 2Check PPC recoveries are within 50–200%.
  3. 3Review sample results against the endotoxin limit and record them.
In LimsPulse · Calibration Management

Keep every Endotoxin reader in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how