LimsPulse
Spectroscopy

Raman spectrometer

Shines a laser on a sample and measures the tiny fraction of light scattered with a shifted wavelength. Gives a sharp molecular fingerprint through glass and plastic, ideal for raw-material identity and polymorph checks.

  • Pharmaceutical
  • Biotech
  • Forensics & security
  • Materials science
  • Semiconductors
  • Art conservation
Photo of a Raman spectrometer
Photo: Ijon, CC BY-SA 4.0 (cropped), via Wikimedia Commons
Follow your site SOP
This is a typical operating sequence for learning. Your instrument model and approved SOP take precedence.
STAGE 1

Before you start

  1. 1Check the calibration status label and that the daily verification has passed.
  2. 2Check the laser interlock, the nose cone or vial holder and the probe window are clean and fitted.
  3. 3Confirm the approved method and library for the material are selected.
  • Only trained, authorised users may operate a Class 3B laser instrument.
STAGE 2

Daily verification

  1. 1Run the built-in or external wavenumber check, usually with a polystyrene or cyclohexane standard.
  2. 2Confirm the band positions are within the SOP tolerance and record the result.
STAGE 3

Identity testing through the bag

  1. 1Select the material method; scan or type the container barcode.
  2. 2Hold the nose cone flat and firmly against the clear inner bag, on a region without labels or folds.
  3. 3Start the measurement; keep the unit still until it finishes.
  4. 4Read the pass/fail result and record it against the container.
  5. 5Fail or 'indeterminate' results go to QC for investigation and full testing.
  • Never point the laser at eyes or skin, or at reflective surfaces.
  • Dark, coloured or heat-sensitive materials can burn: use the low-power setting or a validated alternative test.
  • Do not test through opaque, foil or paper layers.
STAGE 4

Shutdown

  1. 1Switch off the laser and close the software.
  2. 2Clean the nose cone or vial holder with a lint-free tissue.
  3. 3Dock handheld units for charging and complete the usage log.
In LimsPulse · Calibration Management

Keep every Raman in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how