Gel Permeation / Size-Exclusion Chromatography (GPC / SEC)
Separates molecules by their size in solution rather than by chemistry, so large molecules elute first. Used for polymer molecular-weight distribution and for protein aggregates in biologics.
- Pharmaceutical
- Biopharmaceutical
- Polymers & plastics
- Food (polysaccharides)
- Cosmetics
- Chemicals
GPC / SEC calibration procedures
GPC / SEC has no instrument calibration of its own in most labs. Its performance is controlled through qualification (below) and the system suitability or acceptance checks of each test that uses it.
Instrument qualification (DQ · IQ · OQ · PQ)
- 01
DQ — Design qualification
Defines solvent compatibility, temperature range, detectors (RI, UV, MALS), molecular-weight range and software needs.
- 02
IQ — Installation qualification
Confirms components, columns, software and utilities are installed and documented correctly.
- 03
OQ — Operational qualification
Tests flow accuracy and precision, oven temperature, injector precision and detector noise, drift and linearity.
- 04
PQ — Performance qualification
Runs calibration standards and a reference material to show the calibration curve and results are reproducible for routine use.
Keep every GPC / SEC in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.