LimsPulse
Microbiology & sterility

Colony counter

Helps the analyst count colonies on agar plates and membranes accurately, either by a lit magnifier with a counting pen (manual) or by a camera and image analysis software (automated).

  • Pharmaceutical
  • Biotech
  • Food & beverage
  • Water testing
  • Cosmetics
  • Research laboratories
Photo of a Colony counter
Photo: Srini297, CC BY-SA 4.0 (cropped), via Wikimedia Commons
TROUBLESHOOTING

Common problems, causes and fixes

Automated count much higher than manual count
Likely causes
  • • Debris, bubbles or precipitate counted as colonies
  • • Detection threshold too low
What to do
  • • Review the image and edit marks
  • • Re-set the detection parameters within the validated range
  • • Filter or dilute samples that leave particles
Automated count lower than manual count
Likely causes
  • • Small or pale colonies missed
  • • Merged colonies counted as one
  • • Poor lighting
What to do
  • • Adjust lighting and threshold within the validated settings
  • • Use the separation tool or count manually
Pen does not register counts
Likely causes
  • • Pen tip worn or cable loose
  • • Battery low
What to do
  • • Replace the pen tip or battery
  • • Check the connection
Different analysts get different counts
Likely causes
  • • Unclear rules for spreading or merged colonies
  • • Training gaps
What to do
  • • Define counting rules in the SOP
  • • Run periodic analyst comparison counts
In LimsPulse · Calibration Management

Keep every Colony counter in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how