LimsPulse
Bioanalytical

Automated cell counter

Counts cells and measures their viability automatically, usually by imaging a stained sample in a disposable or reusable counting chamber. It replaces manual haemocytometer counting for routine cell culture and bioprocess work.

  • Biotechnology
  • Biopharmaceutical
  • Cell and gene therapy
  • Academic research
  • Vaccine manufacture
  • Regenerative medicine
Photo of a Automated cell counter
Photo: TimVickers, Public domain (cropped), via Wikimedia Commons
Follow your site SOP
This is a typical operating sequence for learning. Your instrument model and approved SOP take precedence.
STAGE 1

Before you start

  1. 1Check the counter is within its bead verification due date.
  2. 2Select the validated protocol for the cell type (stain, dilution factor, size gates).
  3. 3Prepare stain and check it is in date and free of precipitate; filter trypan blue if crystals are present.
  • Handle cell samples in a biosafety cabinet as required by your site biosafety rules.
STAGE 2

Counting

  1. 1Mix the cell suspension gently but thoroughly (pipette up and down or invert) just before sampling.
  2. 2Mix sample and stain in the ratio set in the protocol.
  3. 3Load the chamber at once and insert it in the counter.
  4. 4Run the count within a few minutes of staining.
  5. 5Review the image overlay to check that cells, clumps and debris are classified sensibly.
  6. 6Repeat counts as required (often two or three) and report the mean.
  • Do not leave cells in trypan blue for long; viability falls over time and dead counts rise.
STAGE 3

Shutdown

  1. 1Discard disposable slides into biological waste or clean reusable chambers as directed.
  2. 2Export or archive results and images.
  3. 3Wipe the slide port with a lint-free cloth and suitable disinfectant.
In LimsPulse · Calibration Management

Keep every Cell counter in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how