LimsPulse
Microbiology & sterility

Airborne particle counter

Counts and sizes airborne particles, typically at ≥0.5 µm and ≥5.0 µm, by measuring light scattered as each particle passes through a laser beam. Used to classify clean rooms and to monitor non-viable particles in Grades A–D.

  • Pharmaceutical
  • Biotech
  • Medical devices
  • Semiconductors
  • Hospitals
  • Aerospace
Photo of a Airborne particle counter
Photo: Panek, CC BY-SA 4.0 (cropped), via Wikimedia Commons
WORKING PRINCIPLE

How Particle counter works

Air is drawn through an isokinetic probe into a sensor where a laser diode lights a small view volume. Each particle scatters light in proportion to its size; a photodetector converts each flash into an electrical pulse. Pulse height is compared with threshold voltages set during calibration with certified polystyrene latex (PSL) spheres, and pulses are counted in each size channel. Count ÷ volume sampled = particles per m³.

FLOW

The path of a sample

  1. 01

    Sample inlet

    An isokinetic probe takes air at the same speed as the surrounding unidirectional flow.

  2. 02

    Flow control

    A pump and flow sensor hold the sample flow at the nominal rate, e.g. 28.3 L/min.

  3. 03

    Illumination

    Particles pass one at a time through a laser beam in the sensor.

  4. 04

    Scatter detection

    Scattered light is collected onto a photodetector and turned into pulses.

  5. 05

    Sizing & counting

    Pulse heights are sorted into size channels using thresholds set with PSL spheres.

  6. 06

    Report

    Counts are converted to particles per m³ and compared with grade limits.

COMPONENTS

Main parts and what they do

1

Isokinetic sample probe

Inlet shaped to match the air speed of unidirectional flow.

2

Sample tubing

Connects remote probes to the sensor; as short and straight as possible.

3

Laser diode and optics

Light the view volume and collect scattered light.

4

Photodetector and pulse analyser

Convert light pulses to signals and sort them into size channels.

5

Pump and flow sensor

Hold the sample flow at the nominal rate; checked at calibration.

6

Exhaust HEPA filter

Cleans the air leaving the counter so it does not contaminate the room.

7

Display, memory and printer / software

Set up locations and volumes, store results and export them.

8

Purge (zero count) filter

HEPA filter fitted to the inlet to confirm the counter reads zero.

KEY CONCEPTS

Terms every analyst should know

Cumulative counts

Results are reported as particles equal to or larger than a size (e.g. ≥0.5 µm), so the ≥0.5 µm count includes the ≥5.0 µm particles.

Isokinetic sampling

Sampling at the same air speed as the surrounding flow, so large particles are not over- or under-collected.

Counting efficiency

The fraction of particles of a given size that the counter detects. ISO 21501-4 typically expects about 50% at the minimum detectable size and close to 100% at 1.5–2 times that size.

Coincidence loss

At very high concentrations two particles may be in the beam at once and counted as one larger particle. The maximum concentration is stated by the manufacturer.

Sample volume

ISO 14644-1 sets a minimum volume per location so that at least 20 particles could be counted at the class limit; for Grade A at ≥5.0 µm this leads to sampling 1 m³.

Tubing losses

Particles ≥5.0 µm settle in long or bent tubing. Annex 1 expects tubing length and bends to be minimised and justified.

In LimsPulse · Calibration Management

Keep every Particle counter in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how