LimsPulse
Volumetric & glassware

Micropipette (Piston-Operated Pipette)

A hand-held, piston-operated device that measures and dispenses small liquid volumes, typically 0.1 µL to 10 mL, using disposable tips. Used for standards, dilutions, microbiology and bioassays.

  • Pharmaceutical
  • Biotech
  • Clinical diagnostics
  • Universities
  • Food & beverage
Photo of a Micropipette (Piston-Operated Pipette)
Photo: Microrao, CC BY-SA 4.0 (cropped), via Wikimedia Commons
TROUBLESHOOTING

Common problems, causes and fixes

Drop forms at the tip or volume falls
Likely causes
  • • Tip not fitted tightly
  • • Worn seal or O-ring
  • • Damaged tip cone
What to do
  • • Use the correct tip and fit it firmly
  • • Replace the seal
  • • Replace the tip cone
Delivered volume consistently low
Likely causes
  • • No pre-wetting
  • • Tip immersed too deep or aspiration too fast
  • • Pipette warmer than liquid
What to do
  • • Pre-wet tips
  • • Improve technique
  • • Equilibrate pipette and liquid
Poor precision
Likely causes
  • • Inconsistent plunger speed
  • • Holding the pipette at an angle
  • • Hand warming the pipette
What to do
  • • Train for a steady rhythm
  • • Aspirate vertically
  • • Do not hold the pipette for long periods; rest it on the stand
Plunger stiff or jerky
Likely causes
  • • Dried liquid in the shaft
  • • Damaged piston
What to do
  • • Clean and grease as instructed
  • • Send for service
In LimsPulse · Calibration Management

Keep every Micropipette in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how