Bioanalytical
CO₂ incubator
A warm, humid chamber that holds mammalian cell cultures at body temperature (usually 37 °C), high humidity and a set CO₂ level (usually 5%), so the culture medium stays at the right pH.
- Biotechnology
- Biopharmaceutical
- Cell and gene therapy
- IVF clinics
- Academic research
- Vaccine manufacture
Follow your site SOP
This is a typical operating sequence for learning. Your instrument model and approved SOP take precedence.
STAGE 1
Before you start
- 1Check the incubator is within its verification due date and the display shows set temperature and CO₂.
- 2Check CO₂ cylinder pressure and that the auto-changeover (if fitted) is set.
- 3Check the water pan is filled with sterile purified water.
- Secure CO₂ cylinders upright with a chain or strap.
STAGE 2
Use
- 1Disinfect gloves and vessel surfaces before placing cultures inside.
- 2Place flasks with vented caps (or caps loosened) so gas can exchange.
- 3Open the door as briefly as possible and not more often than needed.
- 4Record entries and any alarms in the logbook or monitoring system.
- Do not overload shelves; air must circulate around vessels.
STAGE 3
After use / end of day
- 1Wipe spills at once with a suitable disinfectant, then sterile water.
- 2Check the display values have recovered after the last opening.
In LimsPulse · Calibration Management
Keep every CO₂ incubator in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.