Bioanalytical
CO₂ incubator
A warm, humid chamber that holds mammalian cell cultures at body temperature (usually 37 °C), high humidity and a set CO₂ level (usually 5%), so the culture medium stays at the right pH.
- Biotechnology
- Biopharmaceutical
- Cell and gene therapy
- IVF clinics
- Academic research
- Vaccine manufacture
PREVENTIVE MAINTENANCE
Maintenance checklist
Daily
- Check and record temperature and CO₂ display values
- Check water pan level and alarm status
Weekly
- Check the water pan for contamination; replace water as per SOP
- Wipe door gasket and inner glass door
Monthly
- Clean and disinfect the interior, shelves and water pan
- Check CO₂ against an independent calibrated analyser if required by SOP
Every 6–12 months
- Verify temperature (with mapping) and CO₂ with calibrated instruments
- Run a decontamination cycle
- Replace CO₂ inlet and HEPA filters as the manual advises
SAFETY
Safety precautions
Before you work on the CO₂ incubator
- CO₂ is an asphyxiant at high concentrations. Ensure adequate room ventilation and check for leaks.
- Secure gas cylinders and use the correct regulator.
- Treat cultures and spills according to their biosafety level.
- High-temperature decontamination cycles make surfaces very hot; let the chamber cool before opening.
In LimsPulse · Calibration Management
Keep every CO₂ incubator in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.