LimsPulse
General lab & storage

Ultrasonic bath (sonicator)

A stainless-steel tank of water vibrated by ultrasound. It is used to dissolve and extract samples, degas mobile phases and clean small glassware.

  • Pharmaceutical
  • Chemicals
  • Food testing
  • Electronics
  • Jewellery and precision cleaning
Photo of a Ultrasonic bath (sonicator)
Photo: Karelj, Public domain (cropped), via Wikimedia Commons
Follow your site SOP
This is a typical operating sequence for learning. Your instrument model and approved SOP take precedence.
STAGE 1

Preparation

  1. 1Fill the tank with purified water to the marked fill line (usually about two-thirds full). A drop of surfactant can improve energy transfer.
  2. 2Degas fresh water by running the bath empty for a few minutes.
  3. 3Check the bath temperature and, if the method requires, add ice or wait for it to cool.
  • Never run the bath without water; the transducers can be damaged.
STAGE 2

Sonicating samples

  1. 1Place flasks in the basket or rack so they do not touch the tank floor.
  2. 2Make sure the liquid level in the flask is at or below the bath water level.
  3. 3Set the time stated in the method and start; shake flasks by hand at intervals if the method says so.
  4. 4After sonication let solutions cool to room temperature before making up to volume.
  • Do not put flammable solvents directly into the tank.
  • Stopper flasks loosely or cover them to avoid splashing and evaporation.
STAGE 3

After use

  1. 1Switch off, drain the water and wipe the tank dry.
  2. 2Record use in the equipment log if required by your SOP.
In LimsPulse · Calibration Management

Keep every Sonicator in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how