LimsPulse
Particle & solid-state

Optical (Light) Microscope

Magnifies particles so their size, shape and crystal habit can be seen and measured with a calibrated eyepiece graticule or camera. Used for particle size, morphology and identification checks.

  • Pharmaceutical
  • Microbiology
  • Materials
  • Food
  • Forensics
  • Education
Photo of a Optical (Light) Microscope
Photo: Kitmondo Marketplace, CC BY 2.0 (cropped), via Wikimedia Commons
TROUBLESHOOTING

Common problems, causes and fixes

Image blurred
Likely causes
  • • Dirty lens or slide
  • • Wrong cover slip thickness
  • • Oil on a dry objective
What to do
  • • Clean optics
  • • Use the correct cover slip
  • • Clean the objective with lens tissue
Uneven illumination
Likely causes
  • • Condenser not centred
  • • Lamp misaligned
What to do
  • • Set Köhler illumination
  • • Re-align the lamp
Particles all clumped
Likely causes
  • • Poor dispersion
  • • Wrong mounting fluid
What to do
  • • Disperse more gently and use less sample
  • • Choose a fluid with suitable wetting
Size results differ between analysts
Likely causes
  • • Different particle selection
  • • Graticule not calibrated
What to do
  • • Use a defined field-scanning pattern
  • • Calibrate and document for each objective
In LimsPulse · Calibration Management

Keep every Optical microscope in control: usage logs, calibration and maintenance schedules, and automatic blocking of overdue instruments.

See how