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Lab glossary

Mass extinction coefficient

The absorbance of a 1 mg/mL protein solution in a 1 cm cell at a given wavelength, in mL mg⁻¹ cm⁻¹. Used with the Beer–Lambert law to calculate protein concentration from A280.

  • Also: absorptivity
  • Also: E 0.1%
  • Also: A280 of 1 mg/mL

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