Lab glossary
Mass extinction coefficient
The absorbance of a 1 mg/mL protein solution in a 1 cm cell at a given wavelength, in mL mg⁻¹ cm⁻¹. Used with the Beer–Lambert law to calculate protein concentration from A280.
- Also: absorptivity
- Also: E 0.1%
- Also: A280 of 1 mg/mL