Lab glossary
Reference wavelength
A second wavelength at which the analyte does not absorb (for example 630 nm in a 450 nm ELISA). Subtracting it corrects for plate scratches and other non-specific optical effects.
- Also: dual-wavelength reading
A second wavelength at which the analyte does not absorb (for example 630 nm in a 450 nm ELISA). Subtracting it corrects for plate scratches and other non-specific optical effects.