Lab glossary
Passive reference dye
A fluorescent dye in a qPCR mix that does not take part in the reaction. The reporter signal is divided by it to correct for small differences in volume and optics between wells.
- Also: ROX
- Also: reference dye
A fluorescent dye in a qPCR mix that does not take part in the reaction. The reporter signal is divided by it to correct for small differences in volume and optics between wells.